• dmpk

Biomarkers and Immunology

Gene sequence or analysis (DNA / RNA)

 Gene expression, mutation, regulation

  • Deep sequencing
  • RNA sequencing
  • Cancer hotspot panel
  • Expression profiling
  • high content analysis
  • PCR / RT-PCR / qPCR

 Apoptosis analysis

  • Cell death Elisa (CDE)
  • TUNEL
  • Click-iT® EdU
  • Enzyme analysis
  • DNA Gel electrophoresis
  • Caspase activation assay

 Protein expression and modification  

  • Western blot analysis
  • MSD
  • ELISA
  • AlphaLISA/AlphaScreen
  • ELISpot
  • Histology and Immunohistochemistry
  • FACS

 Cytokine level

  • ELISA
  • MSD
  • Luminex
  • FACS

Histology

  • Frozen tissue block preparation
  • Paraffin block preparation
  • TMA
  • Sectioning
  • DNA/RNA extraction from frozen or paraffin embedded tissue samples
  • Histological staining including all special histo-chemical staining
  • Immunohistochemistry on frozen or paraffin sections
  • Single, double or multiple antigen-antibody labeling
  • Enzyme/fluorescent labelling

Immune cell activation analysis on PBMC or lymph tissue

  • T/B cell function assay
  • FACS (CD markers)
  • Cytokine production

Other analysis

  • Hematology (CBC count)
  • Blood chemistry (full panel)
  • Urine analysis (volume, Osmolality or chemistry…)
  • Fecal electrolyte, lipid extract and analysis (cholesterol and bile acid, P, Na, Cl, or Ca)
  • CSF (including sample from small rodent) analysis
  • BAF (body aspirate fluid) analysis