Enhancing In Vivo Efficiency Through Automation and Optimisation
End-to-end Automation: Redefining Speed and Quality in Bioanalytical Workflows
High-throughput, high-accuracy in vivo efficiency in sample processing is central to preclinical and clinical research. At Pharmaron, we’ve designed a fully automated, end-to-end (E2E) pipeline for bioanalytical workflows that improves efficiency, minimizes error, and accelerates data delivery.
This automation-first strategy integrates microsampling, liquid handling robotics, Python-based scripting, and real-time LC-MS/MS interfacing to reduce manual intervention by up to 70%, ensuring consistent data quality and turnaround times.
In Vivo Efficiency Through End-to-End Automation
Streamlined Processing Means Faster, Better Data
Traditional in vivo pharmacokinetic (PK) workflows involve manual steps that are time-consuming and prone to human error. Pharmaron’s E2E process optimization addresses these challenges using a combination of:
- Python scripting for batch creation, data extraction, and report formatting
- Automated sample handling via the Bravo Liquid Handler
- Load-ahead sample injection using CTC/Exion systems
Together, these innovations reduce sample preparation time by over 70% (see Table 1 in the source) while improving reproducibility and quality assurance.
Maximizing Scientific Return with Minimal Animal Use
Following the 3Rs—Reduction, Refinement, and Replacement—Pharmaron implements microsampling and high-efficiency scripting to reduce the number of animals required and enhance data quality. For instance, using only 5µL of plasma, serial sampling from rodents yields robust PK profiles, aligning with both ethical standards and scientific excellence.
In Vivo Efficiency Driven by End-to-End Automation Across the Pipeline
Batch Building with Python
Manual run list creation is replaced by scripts that convert animal, dose, and timepoint data into batch files compatible with Sciex Analyst software. This ensures consistency and saves hours per study.
Liquid Handling with Bravo Platform
The Bravo Automated Liquid Handler executes multi-plate protein precipitation, supernatant transfer, and dilution tasks with unmatched precision, reducing manual pipetting errors and operator time.
Smart Compound Tuning
DiscoveryQuant automation, enhanced by custom scripts and SQL database integration, facilitates faster and more accurate compound tuning, streamlining both Quicktune and ChromaTune stages.
In Vivo Efficiency in Data Processing & PK Reporting
Data Flow Optimization
Vendor software output is transformed through scripted pipelines into standardized report formats, ensuring seamless transfer to WinNonlin for PK parameter analysis. This eliminates transcription errors and compresses reporting timelines.
Predictive Modeling for Faster Decisions
Observed and predicted PK data are visualized using automated scripts that produce ready-to-use XY plots and final parameter outputs, accelerating insights and decision-making.
Why It Matters: Speed, Accuracy & Fewer Animals
Pharmaron’s E2E system isn’t just about technology. It’s about:
- Reducing processing time per compound by over 70%
- Ensuring reproducibility in every PK report
- Meeting turnaround expectations without compromising on quality
- Supporting regulatory goals for reduced animal use
References:
Download the full poster to see how this automated in vivo workflow delivers reliable results, faster.