Biophysics
Biophysical Assays
Pharmaron’s biophysical assays team offers a wide range of cutting-edge binding assays across multiple modalities to accelerate and support drug discovery programs. With strong expertise in designing and performing both routine and bespoke biophysical assays, the team supports integrated drug discovery, full-time equivalent, and fee-for-service projects.
Our state-of-the-art biophysical assay technologies support all phases of drug discovery, including protein quality assessment, hit identification, hit confirmation, orthogonal validation, and in-depth characterization of lead candidates.
We specialize in developing customized biophysical assays for mechanistic characterization. Our broad range of services can be applied to both small and large molecule drug discovery.
Our no-wait time approach and industry-leading turnaround times ensure a rapid transition from early-stage discovery to candidate optimization, advancing our clients’ projects with unparalleled precision, efficiency, and scientific rigor.
Capabilities
Binding Interactions
- Fragment/small molecule/antibody screening
- In-depth characterization of molecular interactions
- Accurate determination of binding kinetics (ka, kd) & affinities (KD)
- Affinity measurement in solution
- Ligand-induced protein stabilization and destabilization
- Bespoke mechanism of action assays
- Binary and ternary complex characterization (protein degraders, molecular glues, etc.)
Biophysical Techniques
- Biacore Surface Plasmon Resonance: 8K and S200
- Biolayer Interferometry (BLI)
- Microscale Thermophoresis (MST)
- Thermal Shift Assay
- Differential Scanning Fluorimetry (DSF)
- NanoDSF
- Affinity binding analysis coupled with LC/MS
- Intact mass analysis for covalent binder
- Isothermal Titration Calorimetry (ITC)
- Affinity-selection mass spectrometry via Orbitrap Astral
Case studies showing the potential of biophysical techniques in advancing drug discovery programs.
