High-Throughput Screening

Scientist in a lab coat works at a computer within a high-tech lab, surrounded by advanced machinery, showcasing Pharmaron's high-throughput screening service suite for efficient and automated research

High-Throughput Screening Service

Pharmaron supports drug discovery with state-of-the-art solutions for high-throughput screening services. Our fully automated G3 Explorer high-throughput screening system from Revvity operates with two robotic arms in an enclosed suite with a turnover of about 200 plates per day, amounting to up to 150,000 compounds per week, depending on the assay readout. Plate formats include 96, 384, and 1,536 wells.

The high throughput screening service options include semi- and fully automated workflows to perform biochemical, binding, and cell-based assays with a wide range of readouts ranging, from simple fluorescence to complex high-content imaging.

Clients value the high quality of data generated by the high throughput screening assays, particularly the high reproducibility of our data.

We provide in-house protein production and cell line engineering, enabling swift development of new assays for high throughput screening campaigns.

No waiting time for study initiation, full transparency, state-of-the-art compound management, and high-quality data, along with our highly effective project management system, can accelerate the launch and completion of high-throughput screening campaigns to support our clients’ drug discovery endeavors.

Download our brochure about Pharmaron’s fully automated HTS screening platform

  • Pharmaron library
  • Client library
  • Third-party library
  • Automated compound management
  • Quality control for purity and molecular weight
  • In as little as two weeks depending on assay complexity
  • Protein production
  • Reagent stability control
  • Cell line engineering
  • Quality control parameters:
    • Z’-score
    • MSR (minimum significant ratio)
    • CV (Coefficient of variation)
  • 96, 384, or 1536 well format
  • Pilot run performed with small part of library to confirm QC
  • Throughput up to 150,000 compounds/week for most readouts
  • Up to 200 plates/day for most readouts.
  • A wide range of counter screening options for mechanistic confirmation
  • IC50 dose-response
  • Analogue synthesis
  • Medicinal chemistry support
  • SAR support
High-throughput screening at Pharmaron is performed with the G3 explorer suite.

High-Throughput Screening Readouts

  • Enzyme activity
  • Binding affinity
  • Target engagement
  • Protein-protein interaction
  • Phenotypic readouts in 2D and 3D cellular assays
  • High-content imaging
  • Reporter signal
  • RNA expression
  • Biomarkers
The enclosed suite houses a robot with two arms for automated high-throughput screening.

Assay Platforms Available for High-Throughput Screening Service

  • Affinity-selection mass spectrometry
  • Fluorescence intensity
  • Fluorescence polarization
  • HTRF
  • AlphaLISA, ELISA, MSD
  • Luminescence
  • Microscopic imaging
  • qPCR
  • NanoBRET
  • HiBiT
  • Electrophysiology
  • Second messengers, Ca2+
Part of the high-throughput screening is the plate carousel to move the plate between the robot arms

Library Options

  • Customer library
  • Third-party library
  • Pharmaron libraries
    • Hand-selected diversity compound library
    • Fragment library
    • Kinase inhibitor library
Preparation for high-throughput qPCR screening is performed with the ZephyrG3 automated liquid dispenser.

Key Instrumentation for HTS Screening

  • Explorer G3 integrated lab automation suite
  • Operetta high-content imaging system
  • Orbitrap Astral
  • Envision
  • SynchroPatch 384
  • QuantStudio 5 and 7
  • FLIPR
  • MesoScale Discovery MSD
High throughput screening assays for binding specificity are performed with the cutting-edge orbitrap astral mass spectrometry unit.

Modalities

  • Small molecule compounds
  • Small molecule fragments
  • Covalent compounds
  • Protein degraders
  • Peptides
  • Oligonucleotides
  • Large molecules

Fully Automated High-Throughput Biochemical and Cell-based Screening with Explorer G3 Suite

Scientist in a lab coat observes a computer monitor, highlighting Pharmaron's fully automated high-throughput screening services. Text reads: 'Watch our Robots in Action.'
CTA Biophysical assays