Predicting human metabolic clearance including low turnover compounds using plated hepatocytes supplemented with HepExtend™ medium

Poster Authors:

¹Simon Taylor, ²Yu Wang,²Haofei Luan,²Mandy Xu, ¹Barry Jones

¹Pharmaron UK, Hertford Rd, Hoddesdon, EN11 9BU

²Pharmaron Beijing, 6 Tai-He Rd, Beijing, 100176 China

Here we present the characterization and verification of an in vitro assay using plated human cryopreserved hepatocytes with incubation conditions supplemented with HepExtendTM media. Incorporating a mathematical regression offset approach the assay is capable of characterizing and predicting human metabolic clearance over a wide dynamic range including compounds with low metabolic clearance.

Verified using a range of clinical marker compounds this assay can be used for metabolic IVIVE, human PK predictions and modelling.