Cell Biology
Cell Biology Services
Pharmaron’s cell-based assay platform and toolbox support drug candidate characterization for target engagement and downstream signal pathway analysis, biomarker screening, cellular function and cell phenotype assessment. Our in vitro biology team provides assay development, validation and screening services.
Cell Models
- Human cancer cell lines
- Human normal cell lines
- Mouse cancer cell lines
- Primary cells (PBMC, T Cell, B Cell, Monocyte, etc.)
- Drug-resistant cell lines
- iPSCs
- Engineered cell lines
Capabilities
Assays
- Proliferation Assays
- Click-IT EdU/Brd U Incorporation, CyQuant (DNA content), Cell counting (high content-based analysis), Cell confluence (incuCyte-based analysis), Colony formation (2D/3D)
- Cell Viability Assays
- Cell Titer Glo (2D/3D), Cell Titer Fluor, LDH
Assays
- Apoptosis: Caspase activity/cleaved PARP, Annexin/PI, Western blot-based/image based/FACS-based detection
- Cell Cycle – flow cytometry/high content-image based analysis
- Ferroptosis
- Autophagy
- Cell migration/invasion
- Mitochondrial assays
- Glucose uptake
- Reporter gene analysis
Protein Analysis
- Western blot, automated western blot
- Plate-based readout (assay development to screening)
- Meso scale assay
- ELISA
- HTRF
- AlphaLISA/Alpha SureFire
- In-cell Western
- Immunofluorescence staining
- Flow cytometry/FACS (iQue3)
- Mass spec-based proteomics
Gene Analysis
- Droplet Digital PCR (ddPCR)
- SYBR Green quantitative PCR (qPCR)
- TaqMan qPCR (single, double, triplex)
- Nanostring
- RNAseq
- RNA modification by LC/MS
Studies of Mechanism of Action (MOA)
Target Engagement
- NanoBRET for multiple target classes engagement
- NanoBiT based PPI
- WB-based target engagement within cellular context
- MSD or AlphaLISA-based target engagement within cellular context
- Proteomics-based target engagement or off-target evaluation
Studies of Mechanism of Action (MOA)
Downstream Functional and Signaling Pathway
- Cytokine release analysis (ELISA, AlphaLISA, Luminex)
- Singlet or multiplexed analysis
- Western blot-based signaling pathway exploration
- Immunoprecipitation, coimmunoprecipitation
- Reporter-based cell line gen. & functional assays
- Stress-related pathway exploration
View 5 Case Studies showing how Pharmaron uses High-Content Screening and Real-Time Imaging
