A novel LC-MS/MS assay for the simultaneous measurement of clinically relevant 11-oxyandrogens in human serum
Poster Authors:
Tharun Ponduru, Vidya Sagar Pasumarty, Ayanna Williams, Geena Jose and Mitesh Sanghvi
LC-MS Bioanalysis, Pharmaron US Lab Services, Germantown, Maryland, United States
Clinically relevant 11-oxyandrogens are important biomarkers in reproductive and metabolic diseases. Growing evidence has linked four adrenal-derived androgens to conditions including congenital adrenal hyperplasia (CAH), 21-hydroxylase deficiency (21-OHD) and polycystic ovary syndrome (PCOS).
At WRIB 2026, Pharmaron scientist, Tharun Ponduru, presented a novel multiplexed LC-MS/MS method for simultaneous quantification of all four 11-oxyandrogens in human serum. The method addresses real analytical challenges that have limited routine measurement of these biomarkers until now.
Difficulty in Quantifying Clinically Relevant 11-Oxyandrogens
Measuring these four androgens simultaneously is not straightforward due to physiological concentration ranges and structural similarity. Multiplexed LC-MS/MS assays for 11-oxyandrogens are not yet routinely available, which is a gap this case study addresses.
Without reliable simultaneous quantitation, researchers are left with incomplete data lacking the sensitivity needed for low concentration patient populations.
Capabilities
Our Multiplexed LC-MS/MS Method
This case study’s method pairs supported liquid extraction (SLE) with LC-MS/MS to achieve simultaneous quantification of all four 11-oxyandrogens from a single human serum sample. Through different techniques, our team was able to achieve chromatographic separation and linearity across all four androgens.
What the WRIB 2026 Poster Covers
The poster presents full qualification data for this novel method including precision, accuracy, stability and parallelism. Key areas covered include:
- Intra- and inter-assay precision and accuracy across three batches and four QC levels
- Stability data across bench-top, freeze-thaw and long-term storage conditions
- Dilution linearity in serum across multiple lots
- Parallelism experiment using standard addition in three serum lots
- Inter-analyte interference assessment across all four 11-oxyandrogens
The data support this method as a reliable tool for disease monitoring in CAH, PCOS and 21-OHD — and for research applications where sensitive simultaneous androgen profiling is needed.
Get the Full Poster
Download the WRIB 2026 poster to review chromatography data, full precision and accuracy tables, stability results and the SLE extraction workflow. Pharmaron’s LC-MS bioanalysis team is available to discuss how this method could support your clinical or research program.
References:
- Storbeck KH, O’Reilly MW. The clinical and biochemical significance of 11-oxygenated androgens in human health and disease. Eur J Endocrinol. 2023.
- Fukami M. 11-Oxyandrogens from the viewpoint of pediatric endocrinology. Clin Pediatr Endocrinol. 2022.
- Schiffer L et al. LC-MS/MS assay for profiling classical and 11-oxygenated androgens in saliva. Ann Clin Biochem. 2019.
- ICH M10 Bioanalytical Method Validation
