Radiochemical Assays for Binding and Uptake Studies

Radioligand Binding Testing of Binding Affinity and Kinetics

Pharmaron offers radioligand binding as well as saturation/competition assays by employing radiochemistry tailored for challenging targets such as GPCRs, ion channels, transporters and others. Our expertise encompasses customizable isotope labeling and proprietary cell systems to support both high-throughput screening and in-depth mechanistic studies.

Radiolabeled Uptake Assays

Pharmaron supports radiolabeled uptake assays with quantitative measurement of substrate transport, nutrient uptake, and metabolic fate. We utilize in-house generated stable cell lines, primary tissue homogenates and flexible assay formats for accurate assessment of functional activity and transporter selectivity.

Highlights of Pharmaron’s Radiochemical Assay Services

• Isotope Labeling: Custom synthesis utilizing isotopes including ³H, ¹⁴C, ³⁵S, ³²P/³³P, and ¹²⁵I to address challenging molecular targets and study requirements.
• In-House Engineered Cell Systems: In-house generated stable cell lines for assays targeting GPCRs, ion channels, enzymes, and transporters.
• Kinetic and Mechanistic Profiling: Quantification of binding kinetics, binding affinity, and mechanistic differentiation, including orthosteric vs. allosteric interactions.
• Versatile Assay Platforms: Filtration assays, scintillation proximity assays (SPA), cell-based uptake studies, and metabolic profiling.

Download our flyer to explore how our comprehensive radiochemical assay capabilities can produce valuable insights and reduce timelines in your drug discovery program.