Expression and Purification

High-titer expression of novel biologics can be delivered through both transient and stable mammalian expression systems; while keeping scalability and manufacturability in mind. Pharmaron’s two-step purification processes are customized to each expressed protein, ensuring the highest standard of purity. Leveraging our experience utilizing four different platforms, our team can express even the most difficult of proteins, from therapeutic target proteins to disease-curing modalities. Pharmaron provides comprehensive molecular biology service solutions, to fully support your gene expression needs.

Learn more about Pharmaron’s in vitro biology services

Molecular Cloning

  • Gene synthesis
  • Codon optimization
  • Engineer-in/out any critical residues or sequences
A scientific expert in protective gear uses a pipette in a laboratory to conduct an in vitro disease biology experiment involving liquid samples.

Protein Expression

Four expressions platforms allow our team to express a wide range of proteins, while maintaining the ability to retain any valuable post-translational modifications of the expressed protein or biotherapeutic

  • Mammalian
  • Insect (BEVS)
  • Yeast
  • Bacterial
Two scientists in lab coats and masks conduct an experiment using a pipette in a biosciences lab, with equipment and supplies on the counter.

Protein Purification

Rigorous two-step purification process ensures the highest level of quality required for ensuing analyses

  • Chromatography (Affinity, IEX, SE, etc)
  • Purity analytics