in vitro ADMET

Our team performs efficient screening assays covering the entire in vitro ADMET spectrum including solution properties, drug absorption and transport, metabolic stability and identification, drug-drug interactions and in vitro toxicity.

Solution Properties

• Aqueous solubility
• pKa using Sirius T3 with solid material or DMSO stock
• Partition coefficient (LogD)
• Protein binding/tissue binding (plasma, blood, brain, kidney, skin, lung, liver microsomes and hepatocytes)
• Blood partitioning
• Chemical stability

Drug Absorption and Transport

• Permeability evaluation (PAMPA, Caco-2, MDCK)
• Transporter investigation on substrate and inhibition (P-gp, BCRP, BSEP, MRP2, MRP4, OATP1B1, OATP1B3, OAT1, OAT3, OCT1, OCT2, MATE1, MATE2K, PEPT1)
• ATPase assays (an indirect method to assess interaction between test compound and efflux transporters)