in vitro ADMET

Our team performs efficient screening assays covering the entire in vitro ADMET spectrum including solution properties, drug absorption and transport, metabolic stability and identification, drug-drug interactions and in vitro toxicity.

Solution Properties

  • Aqueous solubility
  • pKa using Sirius T3 with solid material or DMSO stock
  • Partition coefficient (LogD)
  • Protein binding/tissue binding (plasma, blood, brain, kidney, skin, lung, liver microsomes and hepatocytes)
  • Blood partitioning
  • Chemical stability

Drug Absorption and Transport

  • Permeability evaluation (PAMPA, Caco-2, MDCK)
  • Transporter investigation on substrate and inhibition (P-gp, BCRP, BSEP, MRP2, MRP4, OATP1B1, OATP1B3, OAT1, OAT3, OCT1, OCT2, MATE1, MATE2K, PEPT1)
  • ATPase assays (an indirect method to assess interaction between test compound and efflux transporters)

Metabolic Stability and Identification

  • Metabolic stability in liver, intestine, lung and colon using subcellular fractions
  • Long-term hepatocyte culture for low clearance compounds
  • Plasma/blood stability
  • Metabolite identification and profiling
  • GSH/Cyanide trapping
  • Reactivity of Acyl Glucuronides (Ags)

Drug-drug Interactions

  • Substrate evaluation & isoform ID (CYP, UGT, AO, FMO, CES, MAO and XO)
  • Enzyme inhibition (CYP, UGT and non-CYP enzymes)
  • CYP induction (human hepatocytes; HepaRG cells; PXR transfected DPX2 cells)

in vitro Toxicity

  • Liver toxicity package (general and mechanistic toxicity evaluation)
  • Cardiotoxicity
  • Genotoxicity

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