Discovery in vivo PK

Three scientists in white lab coats, blue hair nets, gloves, and masks conduct genetic toxicology experiments in a laboratory. They are working with lab equipment and containers on a bench, with a bright, sterile environment in the background.

Discovery in vivo PK Services

Pharmaron’s discovery in vivo PK service offerings cover screening PK, tissue distribution and excretion, metabolic profiling, metabolite quantification, various other PK models for mechanism studies, endogenous biomarker analysis and PK/PD studies, etc, to support drug discovery projects, to evaluate hits, leads and potential candidates’ properties from in vivo PK perspective.

All in-life work of PK/TK studies is conducted in AAALAC-accredited animal facilities and follows IACUC-approved study protocols.

A scientist wearing a white lab coat, blue hair net, safety glasses, and a face mask operates laboratory equipment with multiple bottles of liquids. The sterile and modern lab setting is equipped for advanced preclinical ADME studies, with various scientific devices and containers visible.

Screening PK

  • Rodents and non-rodents
  • Standard single PK design: full PK, rapid PK, snapshot PK
  • Cassette PK: 3-6 cmpds/route
  • Formulation screening support
  • Dosing routes
    • IV, PO, IP, SC, IM
    • Infusion, intra-colon, intra-duodenum, intra-tracheal, intranasal, dermal
    • IP/SC via ALZET pump
    • Sublingual

Tissue Distribution

  • Drug concentration in tissues
  • Unbound level in tissue
    • Equilibrium dialysis
    • Microdialysis(Kpuu)
  • CNS compounds
    • CSF (rodents & large animal)
    • Brain homogenate
    • Brain slice method

Metabolism and Excretion

  • Metabolite analysis
    • Quantitative analysis of metabolite/parent drug
    • Qualitative analysis
  • Excretion: urine, feces, bile
  • DDI effect
  • Pre-treatment with inhibitor(ABT, Ela)
    • Co-dosing of test articles
    • Pretreatment (Pentagastrin, Famotidine)

in vivo PK Models

  • Portal vein cannulation – dosing and/or sampling
  • Bile duct cannulation – biliary excretion
  • Ussing chamber – intestinal permeability, intestinal absorption, secretion, metabolism
  • PK/PD analysis
    • Systemic exposure
    • Biomarker analysis (LC/MS)
    • Blood chemistry and CBC
    • Pathology

Endogenous Biomarkers

  • Malonyl-CoA, Carnitine, NAD
  • Prostaglandin (PG), Adenosine, Bilirubin, Bile acids (TCA), 
  • Estrone, Estradiol, Dopamine (and their metabolites), etc.

Watch our webinar covering strategy and methodology for predicting human PK evolving from a discovery to a First in Human setting.

A promotional banner for a Pharmaron DMPK Webinar Series titled "Unlocking Discovery and Enabling Clinical Evaluation: Predicting Human Pharmacokinetics and Dose." It highlights the roles of genetic toxicology in drug development, featuring photos of speakers Hannah Jones and Simon Taylor with their titles and affiliations.