Home » Services » in vitro Biology » in vitro Disease Biology

in vitro Disease Biology

A scientist wearing a hairnet, mask, and gloves uses a pipette at a machine labeled "mosquito" in a laboratory. They are conducting preclinical ADME studies, surrounded by lab equipment and bright overhead lighting.

in vitro Disease Biology Services

Our in vitro disease biology team works closely with our partners to evaluate targets, design flexible work flows, and develop and validate assays to meet project specific goals. Pharmaron has strong assay development expertise in key therapeutic areas, including oncology, immunology, IO, metabolic disease, cardiovascular disease,  CNS and pain.

Target-based screening platforms and pathways-centric approaches can be applied to multiple diseases and therapeutic areas.

Capabilities

Target Identification and Validation
High-Throughput Screening Service
Biophysics
Biochemistry
Cell Biology
in vitro Disease Biology
Cell Line Engineering
A lab scene shows various containers with liquids, including a bottle with an orange solution and several clear bottles. A person's gloved hands are seen holding a pipette over a small container. Lab equipment, used for preclinical ADME studies, is arranged on a reflective surface.

Oncology

  • Target validation
  • Drug-resistant cell line development and characterization
  • Cell viability
  • Long-term culture, 2D, 3D
  • 3D spheroid assays (viability/Western blot/qPCR)
  • Apoptosis assays
  • Cell cycle analysis
  • DNA damage (rH2Ax, gene pathways)
  • Angiogenesis
  • Migration/invasion assay; translocation assay
A person wearing blue gloves uses a pipette to transfer liquid into a clear container in a laboratory setting. Various lab equipment, essential for Preclinical ADME studies and genetic toxicology, including other pipettes and bottles, are visible in the background.

Immunology

  • T cell activation/proliferation
  • PBMC assays
  • Whole-blood assays
  • Toll-like receptor
  • cGAS-STING pathway
  • JAK/STAT pathway
  • IRF pathway
  • NF-KB pathway
  • NF-AT pathway
  • PD-1/PD-L1 blockage
  • IDO/TDO/Adenosine receptors
  • Stress pathway exploration
A gloved hand reaches into a laboratory machine used in preclinical ADME studies, with various labeled compartments: Buffer, Flush, Clean, Rinse, and Aux. The machine has a transparent cover and a green indicator light. Nearby, a tray with multiple slots is positioned within the equipment.

Cardiovascular and Metabolic Diseases

  • GSIS (Intact islet/pancreatic beta cell)
  • 3T3-L1 differentiation
  • Fatty acid synthesis assay (C-14)
  • Glucose uptake on different cell models
  • Ephys-based ion channel assays
  • Ephys-recording from primary culture
  • iPS-induced cardiomyocyte
  • Multiple-electrode array recording of cardiomyocyte
A person wearing blue gloves and a white lab coat operates a computer in a laboratory. They are pointing at complex data visualizations displayed on the monitor, including graphs and scatter plots from preclinical ADME studies. A desktop tower is present beside the monitor.

CNS

  • A beta aggregation
  • High content-based reading from primary cultured neuron
  • Chemical-induced neurotoxicity, neurite degeneration model
  • Ephys-based ion channel assays
  • Ephys-recording from primary culture
  • Mechanical induction of neurotoxicity, neurite degeneration model
  • Neurite outgrowth
CTA in vitro Disease Models

Related Services

Researcher of in vivo pharmacology

Learn more about Pharmaron’s animal disease model services

Our in vivo pharmacology team is experienced with disease model establishment and validation for efficacy screening, in vivo pharmacology profiling, PDPK and MOA studies. The team is supported by internal PK/Bioanalytical, ex vivo pharmacology and in vitro biology teams to provide integrated services in various therapeutic areas.
Read More
Blue DNA Helix Molecule

Learn more about Pharmaron’s integrated drug discovery and development services

Pharmaron provides a complete integrated platform from drug discovery to proof of concept (“POC”). Our industry knowledge, strong execution tactics and end-to-end solutions shorten the drug discovery and development cycle.
Read More