Biophysics
Biophysics Services
Pharmaron’s biophysics’ team offers a wide range of cutting-edge biophysical assays across multiple modalities to accelerate and support drug discovery programs. With strong expertise in designing and performing routine and bespoke biophysical assays, the team supports both integrated drug discovery and stand-alone projects.
A suite of state-of-the-art biophysical technologies allows our team to efficiently support all phases of drug discovery programs, including protein quality assessment, hit ID, hit confirmation, orthogonal validation and in-depth characterization of lead candidates.
We specialize in developing customized assays for mechanistic characterization and our broad range of biophysical techniques that can be applied to both small and large molecule drug discovery programs.
Capabilities
Binding Interactions
- Fragment/small molecule/antibody screening
- In-depth characterization of molecular interactions
- Accurate determination of binding kinetics (ka, kd) & affinities (KD)
- Binding assays and affinity measurements in solution
- Ligand-induced protein stabilization and destabilization
- Bespoke mechanism of action assays
- Binary and ternary complex characterization (protein degraders, molecular glues, etc.)
Biophysical Techniques
- Biacore Surface Plasmon Resonance: 8K and S200
- Biolayer Interferometry (BLI)
- Microscale Thermophoresis (MST)
- Thermal Shift Assay
- Differential Scanning Fluorimetry (DSF)
- NanoDSF
- Affinity binding analysis coupled with LC/MS
- Intact mass analysis for covalent binder
Case studies showing the potential of biophysical techniques in advancing drug discovery programs.
